Phosphofructokinase (PFK) exercise was measured utilizing the Phosphofructokinase Action Colorimetric Assay Kit (BioVision, Milpitas, CA, Usa K776-100), in accordance to the manufacturer's guidelines. Briefly, PFK converts fructose-six-phosphate (F6P) and ATP to fructosediphosphate and ADP. ADP is then transformed to adenosine monophosphate (AMP) and NADH in the presence of the substrate and enzy
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